The 1kb dna ladder has thirteen bluntended fragments with sizes ranging from 250bp to 10,000bp. Dna marker blue bp 1kb dna ladder readytouse dna marker bp 1kb dna ladder is designed for use as molecular weight standard for agarose gel electrophoresis features. The pcr dna ladder is ideal for routine agarose gel electrophoresis of pcr products or of any dna fragments sized between 100 bp and 2,000 bp. The ladder consists of bands that are generated from pcr followed by restriction enzyme digestion.
Eleven fragments that range in size from 100bp to 1,000bp in 100bp increments plus a band at 1,500bp. This marker can be used with either agarose or polyacrylamide gels. Examples of the onepot isothermal amplification are q. The ladder consists of bands that are generated from pcr and restriction enzyme digestion of double. I am guessing the two bands showing up in the lanes with samples are the 28s and 18s rna bands but they dont. Pcr markers have six bands of equal intensity of 50, 150, 300, 500, 750 and 1,000bp. The concentration of 1kb dna ladder is 100 ugml 500ul five microlitres 500 ng of the 1 kb dna ladder are mixed with 1ul of blueorange 6x loading dye. The approximate mass of dna in each band is provided 0. The product is supplied in a solution of 10 mm trishcl, ph 8. Use the 1 kb dna extension ladder to size linear doublestranded dna fragments from 500 bp40 kb. Six bands of equal intensity of 50, 150, 300, 500, 750 and 1,000bp. Thirteen bluntended fragments with sizes ranging from 250bp to 10,000bp.
Dna electrophoersis ladder very blurry electrophoresis. Cluster analysis based on similarity was performed by the neighborjoining method using the. The bands of the ladder each contain from 1 to 12 repeats of a 1018. The objective of this study was to characterize 36 isolates of pshv1, previously obtained from. The 500 bp band with higher concentration is distinguished easily from. Jukescantor distances and the neighborjoining method phylip package 7. Is it ok to use dna molecular weight marker in rna.
High dna mass ladder 1 kb plus dna ladder 100 bp dna ladder low dna mass ladder 50 bp dna ladder ultra low range dna ladder. The dna 1 kb ladder is suitable for size determination of doublestranded dna using dna electrophoresis. Combine restriction digestion and dephosphorylation of dna vector in. Each ladder is provided with a tube of 6x blueorange loading dye. The benchtop 1 kb dna ladder promega, madison, wi, usa was used as a dna size marker. Promega manufacturing and delivery systems continue to be fully operational during the covid19. How can i prepare working solution with 1 kb dna ladder. This visual guide allows you to easily compare the differences between dna ladders offered at goldbio in terms of size. Each lane contains the fluorescent ladder cxr, 60400 bases shown in blue. The 1,000bp and 3,000bp fragments have increased intensity relative to the other bands on ethidium bromidestained agarose gels for easy identification. Invitrogen 1 kb plus dna ladder is designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 15000 bp. Molecular weight markers, or ladders, are a set of standards that are used for determining the approximate size of a protein or a nucleic acid fragment run on an electrophoresis gel.
Sigmaaldrich offers a number of directload 1 kb dna ladder products. Dna electrophoersis ladder very blurry nov022010 recently my dna reference ladder invitrogen 1kb plus has been giving me a very blurred smeary result, rather than the distinct bands i am used to and. Promega 1kb dna step ladder molecular weight marker for. Genotypic characterization of psittacid herpesvirus isolates from brazil. Biotium 1 kb dna ladder 100ngul gene target solutions. The ladder is composed of fourteen chromatographypurified. The 1 kb dna ladder is a unique combination of a number of. Ten bluntended dna fragments ranging from 1kb to 10kb in 1kb increments. Dna step ladders contain dna bands of defined sizes and equal intensity with fragment lengths at defined increments.
Dna ladders have defined sizes, and are not intended for use in quantitative analysis. Generuler 1 kb dna ladder 1250 25 x 50 g for 2500 applications, 0. For the convenience, the amounts of the 500 and 1,000 bp. The 1,000bp band appears more intense than all other bands, which are of approximately equal intensity.
Hyperladder 1kb with 1% wv agarose gel, hyperladder 50bp with 1. Contact your local promega branch office or distributor. Diversity and distribution of dna sequences with affinity to. The dna ladder consists of proprietary plasmids which are. Promega 1kb dna ladder molecular weight marker 1kb dna. Promega 1kb dna step ladder molecular weight marker for electrophoresis ten bluntended dna fragments ranging from 1kb to 10kb in 1kb increments. Thirteen bluntended fragments with sizes ranging from. Invitrogen egel 1 kb plus dna ladder is designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 15,000 bp. Please have a look into the related application note for. Promega corporation is a worldwide leader in applying biochemistry and molecular biology to the development of innovative, highvalue products for the life sciences. Generuler 1 kb dna ladder thermo fisher scientific. This ladder has 12 evenly spaced bands ranging from 1kb to 12kb, quick.
Egel 1 kb plus dna ladder thermo fisher scientific. One kb plus dna ladder is composed of 20 highly purified, doublestranded dna bands spanning 100bp to 12,000bp. Characterization of norovirus rna replicase for in vitro amplification. This ladder consists of bands containing 18 repeats of a 1018bp dna fragment.
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